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Part:BBa_K1094000:Design

Designed by: Emil Christian Fischer   Group: iGEM13_UNIK_Copenhagen   (2013-09-03)


Enhanced flavin-binding fluorescent protein (eFbFP)


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI.rc site found at 376


Design Notes

Fluorescence is not easily spotted via fluorescence microscopy using GFP-filters. Thus, a 485nm filter is recommended.


Source

Pseudomonas putida. Enhanced via F37S mutation.

Originally described by: Arnab Mukherjee, Kevin B Weyant, Joshua Walker and Charles M Schroeder (2012): Directed evolution of bright mutants of an oxygen-independent flavin-binding fluorescent protein from Pseudomonas putida. J. Mol. Eng., 6:20.

References